Biology/Microbiology Laboratory

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Biology/Microbiology Laboratory

Based on the Trisakti University policy that focus on the ‘Urban University”, the graduates hoped from  the Program Environmental Technology should be able to solve the Environmental problems, especially the urban environment, as the result of many people activities in the big town as Jakarta.
In operating the education, the Environmental Technology program applies the activities – in class activities, practice in the laboratory, practice drawing/studio, working practice and soon. Especially to the practice implementation, it needs a natural laboratory absolutely to fulfill the students’ need, even in practice, thesis or research facilities to lecturers, and also to serve people.
The biology and microbiology laboratories from the Environmental Technology program have been opened to serve students, lecturers, and public who need those since the even semester 1998/1999. The laboratory activities have been done such as:
  • Introduction to Biology and Microbiology parameters
  • Studying the working technique in the microbiology laboratory
  • Studying the growing of microbe and the factor influenced
  • Studying the roles as bio indicator and biocatalyzator
The aims to do the biology and microbiology laboratory at the Environmental Technology program are for :
1. Serve students to biology and microbiology practice.
2. Serve students to do their thesis
3. Develop science for lecturers around the Environmental Technology Program
4. Supply facilities to serve for public

The Practice Values consists of Some Factors
1. Preparation, consists of discipline and making journal;
2. Quiz, before practicing time
3. Practice, consists of skills, accuracy, quickness, cleanliness, and discipline in working
4. Report, it is an assignment, observation result or report, consists of presentation technique, calculation, discussion and conclusion.
5. Mid Term Test (written)
6. Final Term Test (written)
The values for each factor are based on the table below:
Table 1. Values for Practice
MID TEST (written)
FINAL TEST (written)
5 %
7,5 %
25 %
12,5 %
20 %
30 %
Total 100 %
Passing Requirements
Practical students will pass/finish the Environmental Biology/Microbiology Practice, if their grades based on the table are not less than C.                                                                                    
For those who haven’t completed the requirements based on the table must repeat depending on their needs.                                                                                                                                        
1. Before starting every practice, students have to study every practical procedure (study from the practical direction book) that will be tested in short quiz for 15 minutes.
2. Before practicing, students get the briefing about the analyst procedure to every parameter measured.
3. Students measure and analyze with assistants
4. Take a note to every observation collected in practice
5. Calculate the experiment practice results from the observation results.
6. Make a report that will be submitted to the next practice.

1. Make an application to the Head of Laboratory with the permission of the Head of the Program and the Thesis Supervisor.
2. After Being Agreed by the Head of the Laboratory based on the provided room and equipment, students can start doing the thesis in the laboratory.
3. . Students can apply an application to borrow equipment from the laboratory staffs.
4. If students need the laboratory out of the working hours or holiday, they should apply the application to the Faculty General Division to get the agreement from the Dean.
5. Students should return back or pay for the damaged equipment to the staffs after the    research has been finished, clean the equipment and the place for the research provided in the laboratory.

Once a week the Biology and Microbiology practices at the Environmental Technology Program will be done at certain times, or depend on the need for practice materials such as the observation to microbe, the observation to the behavior of the bio indicator and so on so there is another time out of the practical hours where the students do the observation based on the agreement with the assistants, students and the laboratory staffs who will prepare all students need.

            The practice materials for the environmental biology and microbiology are:
Chapter 1. Microbiology Laboratory Technology
Practice 1
  • Laboratory Techniques
  • Microscope Introduction
  • Sterilization of materials and tools
  • Prepare  Medium
  • Microbe Isolate of solid and liquid (water, land, air)
 Preservation of Conserved Morphology
            (bacteria, fungus, protozoa, virus, microalga)              
Practice 2

1. Check the result of the  purifying isolate
2. Identify the microbe :
Some coloring techniques :
a. Simple Coloring
b. Gram Coloring
c. Negative Coloring
d. Spore Coloring                                                                                                                                                                                   

Duplicating the subculture to the sloping medium NA

Practice 3
1. Identify the microbes: forming pigment (demonstration)
2. Identify the microbes: forming motions (including to study the medium based on the phase)
3. Identify the microbes: the ecology of the microbes

The microbes interaction with abiotic factors :
  • a. the effect of the antibiotic substance
  • b. the effect of  sunlight
  • c. the effect of metal (the mass of oligodinamic)
Duplicating liquid cultures 100 ml

Practice 4
1. Identify the microbes: the microbes ecology
    The microbes interaction with the abiotic factor:
                  d. the glucose content effect
                  e. the temperature effect to the pigmentation and growth
                  f. the effect of the pH growth
                  g. the effect of the oxygen to the growth
                  h. the chemical effect to the growth
2. Duplicate liquid cultures 1000 ml
3. Bio indicator approaching to the community (Plankton & Bentos)

Practice 5
Identification: The microbes interaction with the biotic factors
                  a. The Microbes symbiosis with the taller plants
                       (B.radicicola with R.leguminosorum)
                  b. The  antibiosis around the microbes
                      (B.anthracius dengan pseudomonas aeruginosa)
                  c. The antibiosis around the microbes
                       (E.coli with E.coli)
                  d. Synergism
Practice 6
  • 1. Duplicating the microbes : the techniques of the breeding microbes
      1. in aerobic (liquid cultures, solid cultures)                                                                                             
      2. in anaerobic (demonstration)
            2. Checking the water
            a. E.coli as  bio indicator
            b. Isolation E.Coli with mix cultures
The direct and indirect enumeration
Enumerasi langsung dan tidak langsung

Practice 7
Checking Water

            c. The coloring microbes
            d. The result of the checking water demonstration

The Microbes Quantitation
  • a. The direct quantitation with the Neubauer Hemacytometer
  • b. Cup Calculation
Practice 8
The making of the growing curves
  • a. with a direction calculation
  • b. spectrophotometer calculation

Praktikum 9
Roles of the microbes in nitrogen cycles on the ground
  • a. Nitrogen Fixation
  • b. Nitrification
  • c. Denitrification
  • d. Ammonification
Practice 10

Penerimaan Mahasiswa Baru Trisakti

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Seminar Nasional PAKAR 2018 " Penelitian Multidisiplin untuk Indonesia Lebih Baik " Batas akhir pengumpulan full pa…
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Huge congratulations to Prof Ali Ghufron Mukti who recieved an honorary degree at @covcampus this week @CU_HLS
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Selamat untuk Prof. dr. Ali Ghufron Mukti, M.Sc., Ph.D 💙
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Sedang Hujan, Kampus A Universitas Trisakti (22/11) #usakti #trisakti
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@sein84 Perhatikan..dari Coventry University. *Adm*
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Thursday, 23 November 2017 04:04
Selamat untuk Prof. dr. Ali Ghufron Mukti, M.Sc., Ph.D (Dirjen Sumber Daya Iptek dan Dikti Kementerian Riset, Teknologi dan Pendidikan Tinggi/Rektor Universitas Trisakti) sebagai orang pertama dari Indonesia yang meraih gelar Doktor Kehormatan (Honoris Causa) dari Coventry University, Inggris

#usakti #trisakti
Tuesday, 21 November 2017 07:07
via : Ali Ghufron Mukti Raih Gelar Honoris Causa dari Kampus Inggris.

“Saya pribadi bersyukur atas pemberian gelar kehormatan ini. Tentunya ini merupakan bentuk apresiasi tidak hanya untuk pribadi, tetapi untuk Bangsa Indonesia,” tuturnya dalam siaran pers yang diterima Okezone, Senin (20/11/2017).

Di hadapan Perhimpunan Pelajar Indonesia (PPI) Inggris, Rektor Universitas Trisakti itu berpesan untuk berkontribusi pada peningkatan daya saing melalui pengembangan pengembangan riset, teknologi, dan inovasi.

Baca selanjutnya di

Kementerian Riset, Teknologi dan Pendidikan Tinggi
Monday, 20 November 2017 23:11
Dekan Fakultas Kedokteran Gigi Universitas Trisakti Prof.Dr. Drg. Tri Erri Astoeti, M.Kes membuka BULAN KESEHATAN GIGI NASIONAL 2017 di Rumah Sakit Gigi & Mulut (RSGM) FKG Usakti. Ajak Keluarga Periksa Gigi (GRATIS). Senyum Sehat Senyum Indonesia.

Note: turut hadir Dekan FK Usakti dr. Suriptiastuti, DAP&E,MS

Pepsodent Unilever
#kedokterangigi #rsgm #fkg #kampusb #usakti #trisakti
Monday, 20 November 2017 06:06
Prof. Ir. Asri Nugrahanti, M.S., Ph.D ., IPU & Dr. Ir. Tiena Gustina Amran., IPU menerima sertifikat ASEAN ENGINEERING REGISTER (AER) bersama BKM-PII di Bangkok, Thailand (Nov 2017)

#usakti #trisakti
Saturday, 18 November 2017 08:08
Fakultas Ekonomi dan Bisnis Universitas Trisakti bekerjasama dgn Direktorat Jenderal AHU Kementerian Hukum dan HAM RI ikuti Short Course Human Resource Management Effective Leadership di University of Lincoln & University of Westminster, UK (12 s.d. 18 Nov 2017).
Friday, 17 November 2017 03:03
Pendaftaran Mahasiswa Baru Magister Manajemen Universitas Trisakti (Akreditasi A). Pendaftaran (Gelombang 3) s.d. 18 Nov. Ujian Masuk 21-22 November 2017.

#pascasarjana #magistermanagement #mm #usakti #trisakti
Friday, 17 November 2017 00:12
Short Course Human Resource Management Effective Leadership di University of Lincoln dan University of Westminster, UK (12 s.d. 18 November 2017)

Tampak Foto (ki-ka)
Dr. Eleonora Sofilda, ME (FEB Usakti)
Dr. David Cobham (University of Lincoln)
Prof. Dr. Bambang Soedaryono, AK., MBA. (Dekan FEB Usakti)
Dr. Sarah Hayne (University of Lincoln)

Program Doktor Public Policy Universitas Trisakti
Fakultas Ekonomi dan Bisnis Universitas Trisakti
University of Lincoln
University of Westminster

Kementerian Hukum dan HAM RI #feb #fakultasekonomidanbisnis #usakti #trisakti
Thursday, 16 November 2017 23:11
from - Saksikan Live Streaming Okezone Goes To Campus: Tantangan SDM dan Organisasi dalam Menghadapi Transformasi Era Digital klik: #OkezoneGoesToTrisakti -
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Okezone Goes to Campus is Back!!!
.... kali ini giliran Okezone menyambangi Universitas Trisakti.

Tema Okezone Goes to Campus kali ini sangat menarik guys. Kita bakal bahas soal Tantangan SDM dan Organisasi dalam Menghadapi Transformasi Era Digital.
Untuk narasumbernya sendiri juga keren-keren. Diantaranya:
Prof. dr. Ali Ghufron Mukti, M.Sc., Ph.D (Rektor Universitas Trisakti)
Wijaya Kusuma (Wakil Sekjen Masyarakat Telekomunikasi)
PM Susbandono (Pakar SDM)

Gak ketinggalan, kita juga bakal kongkow bareng Badai Romantic Project juga lho.. So, Don't Miss It our program yaa!
Stay tune di Okezone Goes To Campus: Tantangan SDM dan Organisasi dalam Menghadapi Transformasi Era Digital, Kamis 16 November 2017, mulai pukul 12.30 WIB-14.30 WIB only at

#usakti #trisakti
Thursday, 16 November 2017 03:03
via UPT Perpustakaan Universitas Trisakti - Halo Akademika Usakti🙋
Siapa yang suka selfie atau wefie? Yuk ikutan Selfie-Wefie Contest yang diadain UPT. Perpustakaan Usakti😀
Ketentuan dan caranya mudah banget
1. Peserta adalah mahasiswa aktif USAKTI
2. Cantumkan nama lengkap, NIM, dan fakultas serta caption menarik pada foto tersebut
3. Selfie-Wefie dilakukan di ruang perpustakaan yang berada di lingkungan USAKTI
4. Waktu foto tanggal 1 sampai dengan 23 November 2017
5. Caranya, follow dulu akun instagram perpustakaan Usakti, lalu post dan tag foto kalian dengan hashtag #DiesNatalisUsakti52 #LibraryAndMeJamanNow
6. Upload akan ditutup pada tanggal 23 November 2017
7. Pengumuman pemenang akan diumhmkan di instagram kami pada tanggal 28 November 2017
8. Pembagian hadiah akan dilakukan pada 29 November 2017 di Perpustakaan Usakti
Hadiahnya? Menarik banget loooooh. Yuk buruan foto paling seru dan menangkan hadiahnya😎
Wednesday, 15 November 2017 21:09
Mau Merdeka dari Gigi Berlubang?, Ayo Ajak Keluarga Periksa Gigi! (GRATIS) ke Rumah Sakit Gigi dan Mulut Fakultas Kedokteran Gigi Universitas Trisakti (RSGM FKG Usakti) pada 20-22 November 2017.

Senyum Sehat Senyum Indonesia

#kedokterangigi #rsgm #fkg #kampusb #usakti #trisakti
Wednesday, 15 November 2017 13:01
KONTAN.CO.ID - JAKARTA. Forum Human Capital Indonesia (FHCI) menandatangani perjanjian kerjasama program “Magang Bersertifikat” (Indonesia Student Internship Certification/ISIC) dengan beberapa perguruan tinggi di Indonesia.

Ke-13 perguruan tinggi tersebut adalah Universitas Indonesia, Institute Teknologi Bandung, Universitas Bina Nusantara, Universitas Gunadharma, Universitas Padjadjaran, Telkom University, Universitas Airlangga, STT Nurul Fikri, Universitas Darma Persada, Universitas Gadjah Mada, Institute Pertanian Bogor, Universitas Trisakti, dan Universitas Mercu Buana.

Program ini menargetkan jumlah peserta magang sebanyak 10.000 mahasiswa dari berbagai bidang ilmu. Program ini akan dilakukan dengan konsep 3-2-1, yaitu 3 semester di kampus, 2 semester magang di BUMN, dan 1 semester untuk membuat laporan akhir.

Fakultas Ekonomi dan Bisnis Universitas Trisakti

#fakultasekonomi #fakultas #ekonomi #bisnis #feb #usakti #trisakti
Tuesday, 14 November 2017 23:11
Selasa, 14 Nov 2017 pukul 13.00 - 15.00 WIB ada acara diskusi di gd C lt 9 dalam rangka Hari Habitat Dunia 2017. Pembicara Dirjen Pembiayaan PUPR dan Direktur Eksekutif Kemitraan Habitat. Diselenggarakan oleh Magister Arsitektur FTSP Usakti.

Kementerian Pekerjaan Umum dan Perumahan Rakyat RI
#magister #arsitektur #ftsp
#usakti #trisakti
Tuesday, 14 November 2017 00:12
Fakultas Teknologi Kebumian dan Energi (FTKE) dan Fakultas Hukum (FH) Universitas Trisakti (Usakti) adakan Seminar Nasional dengan Tema " Great Achievements of Different Continents: The Role of Government Regulation in Supporting The Oil and Gas Investment Growth " pada Rabu, 15 November 2017

#ftke #fh #usakti #trisakti
Tuesday, 14 November 2017 00:12
Innalillahi wa inna ilaihi rojiun. Turut berduka cita atas berpulangnya Ibu Siti Hanifah binti Hidayat, Ibunda tercinta dari Prof. Dr. Ir. Dadan Umar Daihani, DEA (Guru Besar FTI, Sekretaris Senat Unversitas Trisakti).

Insya Allah akan dikebumikan di Pemakaman Assalam jalan Sukarno Hatta, berangkat dari Rumah Duka Jl. Reog No 6 Bandung, jam 9.00 pagi (14/11)

Semoga almarhumah Husnul Khotimah dan mendapat tempat terbaik disisi-Nya. Aamiin
Tuesday, 14 November 2017 00:12


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