Biology/Microbiology Laboratory

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Biology/Microbiology Laboratory

INTRODUCTION
Based on the Trisakti University policy that focus on the ‘Urban University”, the graduates hoped from  the Program Environmental Technology should be able to solve the Environmental problems, especially the urban environment, as the result of many people activities in the big town as Jakarta.
In operating the education, the Environmental Technology program applies the activities – in class activities, practice in the laboratory, practice drawing/studio, working practice and soon. Especially to the practice implementation, it needs a natural laboratory absolutely to fulfill the students’ need, even in practice, thesis or research facilities to lecturers, and also to serve people.
The biology and microbiology laboratories from the Environmental Technology program have been opened to serve students, lecturers, and public who need those since the even semester 1998/1999. The laboratory activities have been done such as:
  • Introduction to Biology and Microbiology parameters
  • Studying the working technique in the microbiology laboratory
  • Studying the growing of microbe and the factor influenced
  • Studying the roles as bio indicator and biocatalyzator
 AIMS
The aims to do the biology and microbiology laboratory at the Environmental Technology program are for :
1. Serve students to biology and microbiology practice.
2. Serve students to do their thesis
3. Develop science for lecturers around the Environmental Technology Program
4. Supply facilities to serve for public

PRACTICE EVALUATION SYSTEM
The Practice Values consists of Some Factors
1. Preparation, consists of discipline and making journal;
2. Quiz, before practicing time
3. Practice, consists of skills, accuracy, quickness, cleanliness, and discipline in working
4. Report, it is an assignment, observation result or report, consists of presentation technique, calculation, discussion and conclusion.
5. Mid Term Test (written)
6. Final Term Test (written)
The values for each factor are based on the table below:
Table 1. Values for Practice
No. FACTORS FOR SCORING VALUES
1.
2.
3.
4.
5.
6.
Preparation
Quiz
Practice
Report
MID TEST (written)
FINAL TEST (written)
5 %
7,5 %
25 %
12,5 %
20 %
30 %
Total 100 %
Passing Requirements
Practical students will pass/finish the Environmental Biology/Microbiology Practice, if their grades based on the table are not less than C.                                                                                    
For those who haven’t completed the requirements based on the table must repeat depending on their needs.                                                                                                                                        
 
PRACTICE IMPLEMENTATION
Practice
1. Before starting every practice, students have to study every practical procedure (study from the practical direction book) that will be tested in short quiz for 15 minutes.
2. Before practicing, students get the briefing about the analyst procedure to every parameter measured.
3. Students measure and analyze with assistants
4. Take a note to every observation collected in practice
5. Calculate the experiment practice results from the observation results.
6. Make a report that will be submitted to the next practice.

Thesis
1. Make an application to the Head of Laboratory with the permission of the Head of the Program and the Thesis Supervisor.
2. After Being Agreed by the Head of the Laboratory based on the provided room and equipment, students can start doing the thesis in the laboratory.
3. . Students can apply an application to borrow equipment from the laboratory staffs.
4. If students need the laboratory out of the working hours or holiday, they should apply the application to the Faculty General Division to get the agreement from the Dean.
5. Students should return back or pay for the damaged equipment to the staffs after the    research has been finished, clean the equipment and the place for the research provided in the laboratory.

PRACTICE MATERIALS
Once a week the Biology and Microbiology practices at the Environmental Technology Program will be done at certain times, or depend on the need for practice materials such as the observation to microbe, the observation to the behavior of the bio indicator and so on so there is another time out of the practical hours where the students do the observation based on the agreement with the assistants, students and the laboratory staffs who will prepare all students need.

            The practice materials for the environmental biology and microbiology are:
           
Chapter 1. Microbiology Laboratory Technology
Practice 1
  • Laboratory Techniques
  • Microscope Introduction
  • Sterilization of materials and tools
  • Prepare  Medium
  • Microbe Isolate of solid and liquid (water, land, air)
 Preservation of Conserved Morphology
            (bacteria, fungus, protozoa, virus, microalga)              
        
Practice 2

1. Check the result of the  purifying isolate
2. Identify the microbe :
Some coloring techniques :
a. Simple Coloring
b. Gram Coloring
c. Negative Coloring
d. Spore Coloring                                                                                                                                                                                   

Duplicating the subculture to the sloping medium NA

Practice 3
1. Identify the microbes: forming pigment (demonstration)
2. Identify the microbes: forming motions (including to study the medium based on the phase)
3. Identify the microbes: the ecology of the microbes

The microbes interaction with abiotic factors :
  • a. the effect of the antibiotic substance
  • b. the effect of  sunlight
  • c. the effect of metal (the mass of oligodinamic)
Duplicating liquid cultures 100 ml

Practice 4
1. Identify the microbes: the microbes ecology
    The microbes interaction with the abiotic factor:
                  d. the glucose content effect
                  e. the temperature effect to the pigmentation and growth
                  f. the effect of the pH growth
                  g. the effect of the oxygen to the growth
                  h. the chemical effect to the growth
               
2. Duplicate liquid cultures 1000 ml
3. Bio indicator approaching to the community (Plankton & Bentos)

Practice 5
Identification: The microbes interaction with the biotic factors
                  a. The Microbes symbiosis with the taller plants
                       (B.radicicola with R.leguminosorum)
                  b. The  antibiosis around the microbes
                      (B.anthracius dengan pseudomonas aeruginosa)
                  c. The antibiosis around the microbes
                       (E.coli with E.coli)
                  d. Synergism
               
Practice 6
  • 1. Duplicating the microbes : the techniques of the breeding microbes
      1. in aerobic (liquid cultures, solid cultures)                                                                                             
      2. in anaerobic (demonstration)
            2. Checking the water
            a. E.coli as  bio indicator
            b. Isolation E.Coli with mix cultures
The direct and indirect enumeration
Enumerasi langsung dan tidak langsung

Practice 7
Checking Water

            c. The coloring microbes
            d. The result of the checking water demonstration

The Microbes Quantitation
  • a. The direct quantitation with the Neubauer Hemacytometer
  • b. Cup Calculation
 
Practice 8
The making of the growing curves
  • a. with a direction calculation
  • b. spectrophotometer calculation

Praktikum 9
Roles of the microbes in nitrogen cycles on the ground
  • a. Nitrogen Fixation
  • b. Nitrification
  • c. Denitrification
  • d. Ammonification
Practice 10

           BIOESSAY
 
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اِنّالِلّهِ وَاِنّااِلَيْهِ رَاِجِعُوْنَ

Telah meninggal dunia Dra. Hj. Nidjat Ibrahim, MM (Dosen dan mantan Wakil Dekan III Fakultas Ekonomi dan Bisnis Universitas Trisakti), pada Hari Kamis 6 Desember 2018 jam 22.00 wib. Semoga Allah mengampuni segala dosa beliau, menerima amal ibadah beliau dan mendapatkan tempat terbaik disisi-Nya.

Rumah Duka: Puri Indah blok A 5 no 22, Kembangan - Jakarta Barat. Belakang Fave Hotel - Puri kembangan.

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.
Arsip Tahun 2011 detikcom : Ibu/Umi Nidjat Ibrahim adalah Pasien Cangkok Hati Pertama di Indonesia, pasien perempuan yang merupakan dosen Fakultas Ekonomi dan Bisnis Universitas Trisakti berusia 64 Tahun. Ibu Nidjat yang lahir di Solo ini melakukan transplantasi hati pada 17 Desember 2010 memakan waktu sekitar 13 jam.
.
...pasien transplantasi hati ini sudah 100 persen sehat. Jika dilakukan check up, maka kondisinya 100 persen normal termasuk keadaan hatinya," jelas Dr Tjhang Supardjo,MD,MSurg,FCCS, salah satu dokter yang melakukan transplantasi hati.

Menurut Dr Tjhang, ...pasien ini sudah mengalami sirosis tahap lanjut dan juga kanker hati (hepatoma), sehingga tidak ada jalan lain selain transplantasi.

Operasi transplantasi hati ini dilakukan oleh Tim Transplantasi Hati yang dipimpin oleh Prof. Zheng Shu-sen, MD, PhD, FACS dari The First Affiliated Hospital Zhejiang University, China bersama tim dokter dan perawat Indonesia.

Operasi ini merupakan realisasi dari kerjasama yang dibuat bersama antara RS Puri Indah, Fakultas Kedokteran Universitas Indonesia, RS Cipto Mangunkusumo dengan The First Affiliated Hospital of Zhejiang University, China. https://m.detik.com/health/berita-detikhealth/d-1550895/pasien-cangkok-hati-pertama-di-indonesia-sudah-bugar-lagi
Thursday, 6 December 2018 22:10
Berita duka cita...
Telah meninggal dunia.. Ibu Berliani Batubara (DLB, Mantan Ka. Perpustakaan FH Usakti) Jumat, 30 Nov 2018, pukul 07.20 di RSPAD.

Kami turut berduka dan merasa kehilangan sosok Ibu Berliani yang pengasih dan baik hati. Semoga Keluarga yang ditinggalkan diberi ketabahan. 🙏

Fakultas Hukum Universitas Trisakti #dukausakti
Saturday, 1 December 2018 01:01
Selamat Merayakan Dies Natalis ke-53 Universitas Trisakti (29/11/1965 - 29/11/2018)

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Wednesday, 28 November 2018 23:11
Menyambut #diesnatalis53thusakti : Bu-ibu yang tersayang... yang cantik-cantik.. Rabu dan Kamis, 28-29 November 2018 dari peserta bazar ada yang jualan ikan segar.. dan bisa bakar di tempat.. mereka datang dari #Indramayu, #Pandeglang .. Please🙏 nanti kita makan ikan.. supaya dagangan mereka habis, ga dibawa balik ke kampungnya... mereka adalah UMKM binaan Kementerian Kelautan dan Perikanan RI 👍
Tuesday, 27 November 2018 17:05
Innalillahi wa inna ilaihi roji'un. Allahummaghfirlaha warhamha wa afi'ha wafu'anha. Berita Duka Cita telah Wafat Ibu Dr. Hj. Indah Sulistyawati, MT (Wakil Dekan 2 FTSP Universitas Trisakti) pada hari ini Jum'at, Tgl. 23 November 2018 Pkl. 17.00 WIB di RS Premier Bintaro. Alamat rumah duka Kav DKI Blok 95/23, jl Cempaka III Meruya Utara Jakbar.

Pemakaman di TPU Karet Bivak (24/11) rencana jam 08.00 berangkat dari rumah.

Mari kita do'akan bersama dengan membacakan Surah Al-Fatihah, in shaa Allah almarhumah Husnul Khotimah. Untuk kel besar yg ditinggalkan semoga diberi ketabahan/keikhlasan serta diberi kekuatan. Aamiin Yaa Rabbal'Aalamiin.

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Friday, 23 November 2018 13:01

Professory

Prof. Dr. Wahyudi Wisaksono Faculty of Landscape Architecture and Environmental Technology

Nama Lengkap Prof. Dr. Wahyudi Wisaksono : Pangkat / Golongan Guru Besar IV/e : Fakultas / Jurusan / Bagian Arsitektur…

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